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Daidaita Tsabtace Na Tukwici Da Pipettes, Shigarwa da Amfani da Pipette

1. Ya kamata a lura da maki biyu lokacin pipetting samfurori maras kyau.
a.Tabbatar kurkura sau biyu kafin bututu.
b.Cire ruwan da wuri-wuri bayan an gama buri.

2. Pipetting high danko samfurori
Yi amfani da yanayin jujjuyawar bututu: danna maballin buri/fiddawa zuwa matsayi na biyu (tasha na biyu) lokacin nema da zuwa matsayi na farko (tsayawa ta farko) lokacin fitarwa.Hakanan, ana buƙatar lokacin zama na 3-5 seconds don duka tsotsa da fitarwa.

Tsabtace Tsabtace-2
Tsabtace Tsabtace-3

3. Pipetting high yawa / ƙananan samfurori
Ma'aunin daidaito na pipette ya dogara ne akan canja wurin ruwa mai tsabta.Idan yawancin samfurin ya bambanta da yawa daga yawan ruwa, to, daidaito zai kasance daidai da muni.Sabili da haka, wajibi ne don gano yawan samfurin kafin bututun, sa'an nan kuma daidaita kewayon zuwa samfurin ƙarar samfurin da za a canjawa wuri da yawa.
Alal misali, idan yawancin samfurin shine 1.2 g / cm3 kuma kuna buƙatar canja wurin 300 ul, ya kamata ku saita kewayon zuwa 360 ul.Wannan hanyar daidaitawa ce kawai, amma madaidaiciyar hanyar daidaitawa tana buƙatar amfani da ma'auni ko ma'auni azaman kayan aiki na taimako don ingantacciyar ƙididdiga.

4. Cire samfurori masu girma da ƙananan zafin jiki
Ana buƙatar lura da maki uku lokacin da ake yin bututun samfuran zafin jiki mai ƙarfi / ƙarancin zafi.
Da fari dai, kar a taɓa jika tukwici na pipette kafin bututun.
Abu na biyu, ya kamata a yi amfani da sabon tip pipette ga kowane pipette.
Na uku, a yi sha'awar kuma a zubar da sauri da sauri.

Tips don amfani da pipette

1. Yi amfani da titin pipette mai dacewa: Don tabbatar da daidaito da daidaito, ana bada shawarar cewa ƙarar bututun yana cikin 35% zuwa 100% na kewayon tip pipette.

2. Pipette tip shigarwa: Tare da mafi yawan nau'o'in pipettes, musamman ma'auni na multichannel, dacewa da tukwici ba aiki mai sauƙi ba ne: don cimma kyakkyawan hatimi, hannun rigar pipette yana buƙatar saka hannun rigar pipette a cikin tip sa'an nan kuma ƙara ta hanyar juya shi hagu dama ko girgiza shi baya da baya.Wasu mutane kuma suna amfani da pipette don ƙarfafa tukwici ta hanyar bugun su akai-akai, amma wannan na iya haifar da murdiya na tukwici kuma ya shafi daidaito, ko kuma a cikin yanayi mai tsanani yana lalata pipette, don haka yakamata a guji shi.

3. Tip immersion kwana da zurfin: Dole ne a kiyaye kusurwar tip a cikin digiri na 20 kuma ya kamata a kiyaye shi tsaye;Ana ba da shawarar zurfin zurfin tip kamar haka: Girman Pipette Tukwici zurfin nutsewa 2 µL da 10 µL 1 mm 20 uL da 100 uL 2-3 mm 200 ul da 1000 uL 3-6 mm 5000 µL da 10 ml 6-10 mm

4. Tip rinsing: Don samfuran zafin jiki na al'ada, tip rinsing yana taimakawa wajen inganta daidaito;duk da haka, don samfurori masu girma ko ƙananan zafin jiki, tip rinsing na iya rage daidaito kuma masu amfani ya kamata su kula da musamman.

5. Gudun bututu: Ya kamata a gudanar da bututun a cikin santsi da saurin da ya dace;da sauri saurin buri na iya haifar da samfurin shiga hannun riga, yana haifar da lahani ga piston da hatimi da ƙetare samfurin.

6. Shawarwari don amfani da pipette
1) Kula da daidaitaccen matsayi lokacin da ake yin bututu;kada ku riƙe pipette sosai a kowane lokaci, yi amfani da pipette tare da ƙuƙwalwar yatsa don taimakawa wajen rage gajiyar hannu;canza hannu akai-akai idan zai yiwu.
2) Duba hatimin pipette akai-akai kuma a maye gurbin hatimin idan an same shi yana lalacewa ko yawo.
3) Calibrate pipette sau 1-2 a shekara (ya danganta da yawan amfani).
4) Ga mafi yawan pipettes, piston ya kamata a lubricated kafin da kuma bayan amfani don kula da hatimi;don pipettes kewayon yau da kullun, hatimin kuma yana da kyau ba tare da mai mai ba.


Lokacin aikawa: Juni-03-2019